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Manuscript Title: TweezPal - Optical tweezers analysis and calibration software
Authors: Natan Osterman
Program title: TweezPal
Catalogue identifier: AEGR_v1_0
Distribution format: tar.gz
Journal reference: Comput. Phys. Commun. 181(2010)1911
Programming language: Borland Delphi.
Computer: Any PC running Microsoft Windows.
Operating system: Windows 95, 98, 2000, XP, Vista, 7.
RAM: 12 Mbytes
Keywords: laser tweezers, optical trapping, calibration.
PACS: 87.80.Cc, 07.05.Kf, 05.40.-a.
Classification: 3, 4.14, 18, 23.

Nature of problem:
Quick, robust and user-friendly calibration and analysis of optical tweezers. The optical trap is calibrated from the trajectory of a trapped particle undergoing Brownian motion in a stationary optical trap (input data) using two methods.

Solution method:
Elimination of the experimental drift in position data. Direct calculation of the trap stiffness from the positional variance. Calculation of 1D optical trapping potential from the positional distribution of data points. Trap stiffness calculation by fitting a parabola to the trapping potential. Presentation of X-Y positional density for close inspection of the 2D trapping potential. Calculation of the trap anisotropy.

Running time:
Seconds